Autoclave Sterilization Safety Checklist

Is the autoclave chamber free from debris, residue, and stains? (Visual inspection)

Inspect the door seal for cracks, tears, or deformation. Is the seal pliable and properly seated?

Verify the water level in the reservoir is within the recommended range as indicated by the manufacturer.

Is the drain screen clear of obstructions to ensure proper drainage?

Visually inspect temperature sensors for damage or corrosion.

Verify the pressure gauge is functioning correctly and reads zero when the chamber is at atmospheric pressure.

Confirm items are loaded according to validated procedures, ensuring proper steam penetration.

Verify the autoclave is equipped with a functional air removal system (e.g., vacuum pump or steam-flush pressure-pulse).

Confirm that the selected cycle parameters (temperature, pressure, time) are appropriate for the load being sterilized.

Verify that all safety interlocks (e.g., door locking mechanism) are functioning correctly.

Continuously monitor the pressure and temperature readings throughout the sterilization cycle. Are they within the validated range?

Ensure the cycle time matches the validated sterilization parameters.

Monitor for any alarms or error messages during the cycle. Document any occurrences.

If applicable, monitor steam quality indicators to ensure adequate steam purity.

Verify that condensate is being effectively removed from the chamber during the cycle.

Visually inspect the door seal for any signs of steam leakage during the cycle.

Monitor for excessive pressure fluctuations during the cycle, which could indicate a problem.

Verify that the cycle is progressing through the expected phases (e.g., heating, sterilization, drying).

Verify that biological indicators (BIs) meet the required kill criteria after the cycle. Document results.

Inspect chemical indicators for the appropriate color change, indicating adequate sterilization conditions.

Visually inspect the load for any signs of damage or wetness.

Ensure the drying time is sufficient to prevent condensation on sterilized items.

Verify that the autoclave cycle completed successfully without any errors or interruptions.

Ensure all cycle parameters, BI/CI results, and operator information are accurately documented in the autoclave log.

Allow adequate cooling time before unloading the autoclave to prevent steam burns.

Confirm that unloading procedures are followed to minimize the risk of contamination.

Verify that preventative maintenance is performed according to the manufacturer's recommendations and facility schedule.

Review calibration records for temperature and pressure sensors. Are they within the required tolerances?

Check the door seal replacement schedule. Is it within the recommended timeframe?

Verify that filters are replaced according to the manufacturer's recommendations.

Inspect the pressure relief valve for proper function and ensure it is not obstructed.

Verify the steam trap is functioning correctly to remove condensate from the steam lines.

Monitor the quality of the water used in the autoclave to prevent scale buildup and corrosion.

Verify that the autoclave chamber is cleaned regularly according to the facility's SOP.